Diabetes Mellitus Blog

Treatment phase of stem cell therapy are:
1. Extraction
2. Isolation, analysis and concentration of stem cells in the laboratory
3. Implantation of stem cells
4. Aftercare

Extraction of bone marrow
One of the doctors removed bone marrow from the patient’s hip. This procedure takes approximately 30 minutes in total and first provided anesthesia to the puncture site and then removed with a fine needle approx. 150-200 ml of bone marrow. The injection of anesthesia can be a bit painful, extraction of bone marrow change is barely felt. Following this intervention will determine the date for implantation of bone marrow and soon the patient can go home.

Another method for the extraction of stem cells is the mobilization of stem cells from bone marrow with the help of growth factors. they are first injected into the patient, making the stem cells come from bone marrow into the blood. Then stem cells can be isolated from the blood. This method is used less frequently in XCell-Center, but in some cases may constitute an alternative to bone marrow puncture.
Isolation, analysis and concentration of stem cells in the laboratory

In the lab examines the bone marrow collected in sterile tubes in quality and quantity of stem cells it contains. This was first isolated stem cells apart, using a chromatographic procedure, the red and white blood cells and plasma. The sample is processed under sterile conditions so that the stem cells provided to the patient may become contaminated with viruses, bacteria or fungi. Additionally, before continuing with sample processing, examines that no viral markers such as HIV, hepatitis B and C and cytomegalic, if an outcome in this respect, anyway checks whether the therapy can be performed with the measures appropriate precautions. The entire process is documented and the concentrate of freshly isolated stem cells are released to the administration when they have met all quality criteria.

The purified stem cells are counted and discussed in terms of its vitality. If there are enough vital stem cells, ie more than two million CD34 positive cells with a vitality greater than 80 percent, the concentrated stem cells is able to be administered to the patient. To use the stem cells thus prepared are kept at minus 196 degrees Celsius in liquid nitrogen.